So I finally got a pic of the new cat up on my myspace page check it out if you're interested. So far he's going by the name Tidy Cat, but I'm pretty sure its just a temporary name until we can think of a better one.
He had a wicked run in with Razul yesterday which may have tramatized him some. She loves cats and tends to get a bit hyper so he got a bit freaked and has been hiding up stairs somewhere.
Today I redid much of my resume. Due to the volume (0) of calls comming in I figured it was time to try something new.
Here is how it looks now, in case you know of any place I'd fit in. Also those dolar signes were supposed to be bullet points.
OBJECTIVE
To obtain a position in a laboratory setting that will utilize skills obtained in both academic and industrial (GMP) settings.
EDUCATION
M.S. Microbiology (Pending), University of Connecticut, Farmington, Ct.
B.S. in Microbiology/Biotechnology minor in Chemistry, May 1998Quinnipiac University Hamden, CT
LABORATORY SKILLS
Microbiology:
Growth of bacteria,
Mutant Isolation: amino acid dependent, temperature sensitive, antibiotic sensitive
Bacterial Transformation
Gene Knockout
PCR screen for mutants
Membrane lipid Isolation
Electron Microscopy
Molecular Biology:
ELISA: Molecular Devices Microplate Reader
Gel Electrophoresis: SDS-PAGE (Novex and Phast systems), IsoElectric Foucusing (Novex and Phast systems), Silver Staining, Coomassie Staining analyzed by Molecular Dynamics Personal Densitometer
Western Blotting (SDS-PAGE and IEF) Molecular Dynamics FluorImager 595
Peptide mapping
DNA isolation, Agarose gel electrophoresis, Southern Blotting.
Mass Spec (MALDI-TOF)
Peptide purification: Affinity column, FPLC
Protein Assays: BCA, Bio-Rad
Protein Modifications: RCM, cross-linking
Chemistry:
HPLC: Reverse Phase and Gel permeation, Waters Alliance, Perkin Elmer series 200
FPLC
TLC
UV Spectrophotometry: HP Chemstation
NMR
ACADEMIC EXPERIENCE
UConn Health Center, Farmington, Ct
Graduate Student (08/04-1/07) M.S. degree pending
§ Investigation of the role of phospholipids in the inner membrane of Bacillus subtilis spores.
§ Created mutant strains of knockouts of specific phospholipids.
§ Ran assays to test for growth, sporulation, resistance, and germination of mutant strains.
§ Preformed isolation and analysis of cell and spore membrane phospholipids.
§ Investigated the role of dipicolinic acids and small acid soluble proteins in resistance of spores of B. subtilis.
Quinnipiac College, Hamden, Connecticut
Research Assistant (09/97-04/98)
Investigated of the role of bacterial flagella in attachment to biofilm.
INDUSTRY EXPERIENCE
Pfizer, Inc., Groton, Connecticut
Assistant Scientist II (01/03-08/04)
11/02- 08/04 GMP Clinical Lot release for Monoclonal Antibody
§ Set up GMP laboratory for work on monoclonal Abs (selection, acquisition, and qualification of equipment IQ/OQ/PQ).
§ Routinely ran SDS-PAGE, IEF, UV, Peptide mapping, and SEC for lot release of mAbs. Developed methods for SDS-PAGE, IEF, and UV.
§ Developed, validated, and ran methods for BCA assay for Validation of cleaning methods for liquid dosing manufacture.
§ Issued Test note for release of products.
§ Collaborated with In Process Control group in Bioprocess Research and Development as technical expert to optimize methods.
§ Participated in experimental design.
§ Routinely analyzed data. Routinely prepared written summary of data. Maintained legal notebook.
Assistant Scientist I (08/00-12/02)
01/02-11-02 Small Molecule Antipsychotic drug
§ Developed validated GMP assay for Potency of Small Molecule Antipsychotic.
§ Ran Potency assay for samples (reference materials, test formulations, and stability studies),
§ Validated Purity Assay for Small Molecule Antipsychotic and its impurities/
§ Ran Purity assay for samples (reference materials, test formulations, and stability studies), Developed GPC assay for PLGA in Formulation.
010/00-01/02 Small Molecule Canine Anti-vomiting drug
§ Analyzed Small Molecule drug samples (stability studies, test formulations, reference materials) by Reverse Phase HPLC, Size Exclusion HPLC, and Foreign Matter examinations.
§ Maintained Veterinary Medicine web page.
§ Participated in data analysis.
§ Routinely prepared written summary of data and presented at team meetings. Maintained legal notebook.
08/00-10/00 Feline Erythropoetin Plasmid (DNA)
§ Analyzed Stability Samples Carried out experiments to develop agarose gel electrophoresis method for analysis of plasmid DNA.
§ Worked on development of method for southern blot for host cell DNA.
State University of New York, Stony Brook
Laboratory Assistant (02/00-07/00)
§ Created Custom Peptide sequences using Peptide synthesizer, (Applied Biosystems, 431A).
§ Purified peptides by reverse phase HPLC, and FPLC.
§ Tested purified peptides by Mass Spectroscopy. (MALDI-TOF) (Burker, Protein TOF).
§ Separated His-tagged proteins by nickel column.
§ Performed General Lab maintenance and preparation of common use solutions and lab supplies.
Pfizer, Inc., Groton, Connecticut,
Research Assistant (01/99-01/00) Assigned Employee via Manpower
§ Analyzed Protein Drug samples (reference materials, test formulations, stability samples, and lots for use in clinical trials) by SEHPLC, SDS-PAGE, and IEF.
§ Performed Method Validation for SDS-PAGE.
§ Performed method optimization and preliminary validation for IEF and Western Blotting.
§ Carried out experiments to develop ELISA method for protein drug, including antibody screening/selection, titration of IgG content, and antibody typing.
§ Created Pipetman Calibration Documentation Site utilized by the ARD bG-CSF Team. (Co-Created procedures used for routine maintenance and calibration.)
§ Assisted in developing procedures for calibration and validation of specialized equipment. Created and maintained ARD bG-CSF web page.
§ Participated in experimental design and data analysis.
§ Routinely prepared written summary of data. Maintained legal notebook.
ADDITIONAL TRAINING
Sterility Testing Seminar 6/5/01
Endotoxin Seminar 19/9/01
Bioburden/Antimicrobial effectiveness/PET Seminar 5/14/02
COMPUTER SKILLS
Expertise in Windows 95, NT, XP, & 2000: PerkinElmer Turbochrom, Waters MillenniumÔ and GPC
Software, ImageQuantÔ, Fragment Analysis, Soft Max PRO, Omiga Sequence Analysis Software HTML, Front Page, Adobe (Premier, PhotoShop, Acrobat, Illustrator, Go-Live), Dream weaver, Flash, Word (MS, Perfect), Excel, Netscape, Internet Explorer, and Outlook
Expertise in Mac: Claris Works , Fragment Analysis ,ImageQuantÔ, Fragment Analysis, Soft
Max PRO, Omiga Sequence Analysis Software HTML, Adobe (Premier, PhotoShop, Acrobat, Illustrator, Go-Live), Dream weaver, Flash, Word (MS, Perfect), Excel, Netscape, Internet Explorer, and Outlook
AWARDS
Pfizer Analytical R&G Impact Award 1st quarter 2001
Pfizer Sextant Award for 2001 Veterinary Medicine Full Development
PRESENTATIONS AND PUBLICATIONS
"Development of Quantitative SDS-PAGE for Proteins", by N. Peckham, R. Kitchel, R. Bartkowski, D. Lee, and L. Margulis. Pfizer Animal Health New Technology Presentation (5/27/99) (Also presented to: Animal Health Management 7/14/99, and to Developmental Research Management 8/23/99)
"Quantitative SDS-PAGE for Proteins", by N. Peckham, R. Kitchel, R. Bartkowski, D. Lee, and L. Margulis. Pfizer Analytical Staff Meeting/ Educational Lunch (7/99) and Pfizer Animal Health Educational Lunch (8/17/99)
"An Udder Flow of Info: Creating the bG-CSF Web Page," by R. Kitchel and N. Peckham. Pfizer Developmental Research Year End Celebration (12/14/99)
"Patients Producing Their Own Drug: Pfizer's First Gene Therapy Product in Dev. Res.," by N. Peckham, R. Kitchel, R. Bartkowski, R. Adami, T. Darrington, and L. Margulis. Pfizer Developmental Research Year End Celebration (12/14/99)
"The Characterization of bG-CSF Aggregation by Size Exclusion Chromatography, SDS-PAGE, and Multiangle Laser Light Scattering," by Richard Bartkowski, Ryan Kitchel, Niomi Peckham, and Leonid Margulis. Analytical R&D Poster Session 2000 (2/23/00)
"Patients Producing Their Own Drug: Pfizer's First Gene Therapy Product in Dev. Res.," by N. Peckham, R. Kitchel, R. Bartkowski, R. Adami, T. Darrington, and L. Margulis. Analytical R&D Poster Session 2000 (2/23/00)
"Determining the Long-Term Stability of a Protein Pharmaceutical for Support of a Clinical Dosage Form Nomination," by N. Hay, L. Zhang, K. Williams, T.Darrington, R.Fedechko, C. Speaks, T. Gipp, R. Kitchel, N. Peckham, R. Bartkowski, L. Margulis, and K. Freeman. Analytical R&D Poster Session 2000 (2/23/00)
"Analytical Technology in Protein Purposeful Degradation Studies," by bG-CSF and cG-CSF AR&D Teams. Pharmaceutical Sciences Technology Showcase. (7/25/00)
"EPO-DNA Identity and Purity: State of the Art in Agarose Gel Electrophoresis," by N.R. Peckham, J.A. Lippke, J. Yu, R. Kitchel, L.A. Margulis. Presentation to Pharmaceutical Sciences Management. (8/4/00)
"The Characterization of Protein Aggregation Through Forced Degradation Studies", R. Bartkowski, R. Kitchel, N. Peckham, and L. Margulis. 2000 Colorado Protein Stability Conference, Breckenridge CO. (7/13-14/00)
"Development of an Agarose Gel Electrophoresis Separation Method for feline EPO DNA", N.R. Peckham, J.A. Lippke, R.T. Kitchel, L.A. Margulis. Pfizer Pharmaceutical Sciences Year End Celebration (12/12/00)
"Aggregation of Recombinant bovine Granulocyte Colony Stimulating Factor in Solution", March 2002 Journal of Protein Chemistry R. Bartkowski, R. Kitchel, N. Peckham, and L. Margulis
“Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins.” J Bacteriol. 2006 Jun;188(11):3740-7. Setlow B, Atluri S, Kitchel R, Koziol-Dube K, Setlow P.
He had a wicked run in with Razul yesterday which may have tramatized him some. She loves cats and tends to get a bit hyper so he got a bit freaked and has been hiding up stairs somewhere.
Today I redid much of my resume. Due to the volume (0) of calls comming in I figured it was time to try something new.
Here is how it looks now, in case you know of any place I'd fit in. Also those dolar signes were supposed to be bullet points.
OBJECTIVE
To obtain a position in a laboratory setting that will utilize skills obtained in both academic and industrial (GMP) settings.
EDUCATION
M.S. Microbiology (Pending), University of Connecticut, Farmington, Ct.
B.S. in Microbiology/Biotechnology minor in Chemistry, May 1998Quinnipiac University Hamden, CT
LABORATORY SKILLS
Microbiology:
Growth of bacteria,
Mutant Isolation: amino acid dependent, temperature sensitive, antibiotic sensitive
Bacterial Transformation
Gene Knockout
PCR screen for mutants
Membrane lipid Isolation
Electron Microscopy
Molecular Biology:
ELISA: Molecular Devices Microplate Reader
Gel Electrophoresis: SDS-PAGE (Novex and Phast systems), IsoElectric Foucusing (Novex and Phast systems), Silver Staining, Coomassie Staining analyzed by Molecular Dynamics Personal Densitometer
Western Blotting (SDS-PAGE and IEF) Molecular Dynamics FluorImager 595
Peptide mapping
DNA isolation, Agarose gel electrophoresis, Southern Blotting.
Mass Spec (MALDI-TOF)
Peptide purification: Affinity column, FPLC
Protein Assays: BCA, Bio-Rad
Protein Modifications: RCM, cross-linking
Chemistry:
HPLC: Reverse Phase and Gel permeation, Waters Alliance, Perkin Elmer series 200
FPLC
TLC
UV Spectrophotometry: HP Chemstation
NMR
ACADEMIC EXPERIENCE
UConn Health Center, Farmington, Ct
Graduate Student (08/04-1/07) M.S. degree pending
§ Investigation of the role of phospholipids in the inner membrane of Bacillus subtilis spores.
§ Created mutant strains of knockouts of specific phospholipids.
§ Ran assays to test for growth, sporulation, resistance, and germination of mutant strains.
§ Preformed isolation and analysis of cell and spore membrane phospholipids.
§ Investigated the role of dipicolinic acids and small acid soluble proteins in resistance of spores of B. subtilis.
Quinnipiac College, Hamden, Connecticut
Research Assistant (09/97-04/98)
Investigated of the role of bacterial flagella in attachment to biofilm.
INDUSTRY EXPERIENCE
Pfizer, Inc., Groton, Connecticut
Assistant Scientist II (01/03-08/04)
11/02- 08/04 GMP Clinical Lot release for Monoclonal Antibody
§ Set up GMP laboratory for work on monoclonal Abs (selection, acquisition, and qualification of equipment IQ/OQ/PQ).
§ Routinely ran SDS-PAGE, IEF, UV, Peptide mapping, and SEC for lot release of mAbs. Developed methods for SDS-PAGE, IEF, and UV.
§ Developed, validated, and ran methods for BCA assay for Validation of cleaning methods for liquid dosing manufacture.
§ Issued Test note for release of products.
§ Collaborated with In Process Control group in Bioprocess Research and Development as technical expert to optimize methods.
§ Participated in experimental design.
§ Routinely analyzed data. Routinely prepared written summary of data. Maintained legal notebook.
Assistant Scientist I (08/00-12/02)
01/02-11-02 Small Molecule Antipsychotic drug
§ Developed validated GMP assay for Potency of Small Molecule Antipsychotic.
§ Ran Potency assay for samples (reference materials, test formulations, and stability studies),
§ Validated Purity Assay for Small Molecule Antipsychotic and its impurities/
§ Ran Purity assay for samples (reference materials, test formulations, and stability studies), Developed GPC assay for PLGA in Formulation.
010/00-01/02 Small Molecule Canine Anti-vomiting drug
§ Analyzed Small Molecule drug samples (stability studies, test formulations, reference materials) by Reverse Phase HPLC, Size Exclusion HPLC, and Foreign Matter examinations.
§ Maintained Veterinary Medicine web page.
§ Participated in data analysis.
§ Routinely prepared written summary of data and presented at team meetings. Maintained legal notebook.
08/00-10/00 Feline Erythropoetin Plasmid (DNA)
§ Analyzed Stability Samples Carried out experiments to develop agarose gel electrophoresis method for analysis of plasmid DNA.
§ Worked on development of method for southern blot for host cell DNA.
State University of New York, Stony Brook
Laboratory Assistant (02/00-07/00)
§ Created Custom Peptide sequences using Peptide synthesizer, (Applied Biosystems, 431A).
§ Purified peptides by reverse phase HPLC, and FPLC.
§ Tested purified peptides by Mass Spectroscopy. (MALDI-TOF) (Burker, Protein TOF).
§ Separated His-tagged proteins by nickel column.
§ Performed General Lab maintenance and preparation of common use solutions and lab supplies.
Pfizer, Inc., Groton, Connecticut,
Research Assistant (01/99-01/00) Assigned Employee via Manpower
§ Analyzed Protein Drug samples (reference materials, test formulations, stability samples, and lots for use in clinical trials) by SEHPLC, SDS-PAGE, and IEF.
§ Performed Method Validation for SDS-PAGE.
§ Performed method optimization and preliminary validation for IEF and Western Blotting.
§ Carried out experiments to develop ELISA method for protein drug, including antibody screening/selection, titration of IgG content, and antibody typing.
§ Created Pipetman Calibration Documentation Site utilized by the ARD bG-CSF Team. (Co-Created procedures used for routine maintenance and calibration.)
§ Assisted in developing procedures for calibration and validation of specialized equipment. Created and maintained ARD bG-CSF web page.
§ Participated in experimental design and data analysis.
§ Routinely prepared written summary of data. Maintained legal notebook.
ADDITIONAL TRAINING
Sterility Testing Seminar 6/5/01
Endotoxin Seminar 19/9/01
Bioburden/Antimicrobial effectiveness/PET Seminar 5/14/02
COMPUTER SKILLS
Expertise in Windows 95, NT, XP, & 2000: PerkinElmer Turbochrom, Waters MillenniumÔ and GPC
Software, ImageQuantÔ, Fragment Analysis, Soft Max PRO, Omiga Sequence Analysis Software HTML, Front Page, Adobe (Premier, PhotoShop, Acrobat, Illustrator, Go-Live), Dream weaver, Flash, Word (MS, Perfect), Excel, Netscape, Internet Explorer, and Outlook
Expertise in Mac: Claris Works , Fragment Analysis ,ImageQuantÔ, Fragment Analysis, Soft
Max PRO, Omiga Sequence Analysis Software HTML, Adobe (Premier, PhotoShop, Acrobat, Illustrator, Go-Live), Dream weaver, Flash, Word (MS, Perfect), Excel, Netscape, Internet Explorer, and Outlook
AWARDS
Pfizer Analytical R&G Impact Award 1st quarter 2001
Pfizer Sextant Award for 2001 Veterinary Medicine Full Development
PRESENTATIONS AND PUBLICATIONS
"Development of Quantitative SDS-PAGE for Proteins", by N. Peckham, R. Kitchel, R. Bartkowski, D. Lee, and L. Margulis. Pfizer Animal Health New Technology Presentation (5/27/99) (Also presented to: Animal Health Management 7/14/99, and to Developmental Research Management 8/23/99)
"Quantitative SDS-PAGE for Proteins", by N. Peckham, R. Kitchel, R. Bartkowski, D. Lee, and L. Margulis. Pfizer Analytical Staff Meeting/ Educational Lunch (7/99) and Pfizer Animal Health Educational Lunch (8/17/99)
"An Udder Flow of Info: Creating the bG-CSF Web Page," by R. Kitchel and N. Peckham. Pfizer Developmental Research Year End Celebration (12/14/99)
"Patients Producing Their Own Drug: Pfizer's First Gene Therapy Product in Dev. Res.," by N. Peckham, R. Kitchel, R. Bartkowski, R. Adami, T. Darrington, and L. Margulis. Pfizer Developmental Research Year End Celebration (12/14/99)
"The Characterization of bG-CSF Aggregation by Size Exclusion Chromatography, SDS-PAGE, and Multiangle Laser Light Scattering," by Richard Bartkowski, Ryan Kitchel, Niomi Peckham, and Leonid Margulis. Analytical R&D Poster Session 2000 (2/23/00)
"Patients Producing Their Own Drug: Pfizer's First Gene Therapy Product in Dev. Res.," by N. Peckham, R. Kitchel, R. Bartkowski, R. Adami, T. Darrington, and L. Margulis. Analytical R&D Poster Session 2000 (2/23/00)
"Determining the Long-Term Stability of a Protein Pharmaceutical for Support of a Clinical Dosage Form Nomination," by N. Hay, L. Zhang, K. Williams, T.Darrington, R.Fedechko, C. Speaks, T. Gipp, R. Kitchel, N. Peckham, R. Bartkowski, L. Margulis, and K. Freeman. Analytical R&D Poster Session 2000 (2/23/00)
"Analytical Technology in Protein Purposeful Degradation Studies," by bG-CSF and cG-CSF AR&D Teams. Pharmaceutical Sciences Technology Showcase. (7/25/00)
"EPO-DNA Identity and Purity: State of the Art in Agarose Gel Electrophoresis," by N.R. Peckham, J.A. Lippke, J. Yu, R. Kitchel, L.A. Margulis. Presentation to Pharmaceutical Sciences Management. (8/4/00)
"The Characterization of Protein Aggregation Through Forced Degradation Studies", R. Bartkowski, R. Kitchel, N. Peckham, and L. Margulis. 2000 Colorado Protein Stability Conference, Breckenridge CO. (7/13-14/00)
"Development of an Agarose Gel Electrophoresis Separation Method for feline EPO DNA", N.R. Peckham, J.A. Lippke, R.T. Kitchel, L.A. Margulis. Pfizer Pharmaceutical Sciences Year End Celebration (12/12/00)
"Aggregation of Recombinant bovine Granulocyte Colony Stimulating Factor in Solution", March 2002 Journal of Protein Chemistry R. Bartkowski, R. Kitchel, N. Peckham, and L. Margulis
“Role of dipicolinic acid in resistance and stability of spores of Bacillus subtilis with or without DNA-protective alpha/beta-type small acid-soluble proteins.” J Bacteriol. 2006 Jun;188(11):3740-7. Setlow B, Atluri S, Kitchel R, Koziol-Dube K, Setlow P.
8 Comments:
What's your myspace account name again? (Get a live journal!)
http://www.myspace.com/ryankitchel
pretty easy to remember.
See if you can think of a name for him.
Holy Hell! FBI on your friends page -- the Funk Brokers. The lead singer, the brunette on the left, is Tom's old boss Kimberly!!!
Did he point you to that band or is this a freaky coincidence?
Bleargh, I can't look at your pix because I don't have a myspace account. I guess I'll have to wait until Friday.
Welcome to free game park play free game pc, there have more free game to play, as family feud free game & chess free game.
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